Active Recombinant Mouse Il21 Protein (His18-Ser146), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il21-265M
• Product Overview: Recombinant Mouse Il21 Protein (His18-Ser146) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: His18-Ser146
• Description: Interleukin-21 (IL-21) belongs to the IL-15/IL-21 family, which exerts pleiotropic immune regulations. IL-21 produced primarily by natural killer T (NKT) cells, T follicular helper (TFH) cells and TH17 cells. As a pleiotropic cytokine, IL-21 has been shown to regulate both innate and humoral immunity. It has potent inhibitory activity towards the activation and maturation of granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced dendritic cells (DCs). In B cells, IL-21 has a major role in the development of immunoglobulin responses. In T cells, it is required to facilitate the functional differentiation of several CD4+ T cell subsets. In addition, the ability of IL-21 to enhance the cytotoxic activity of both CD8+ T cells and NK cells makes it as a potential antitumor agent.
• Form: Lyophilized
• Bio-activity: Measure by its ability to enhance IFN gamma secretion in NK-92 cells. The ED50 for this effect is < 6 ng/mL. The specific activity of recombinant mouse IL-21 is > 1.6 × 10⁵ IU/mg.
• Molecular Mass: The protein has a calculated MW of 15.9 kDa.
  The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il21 interleukin 21 [ Mus musculus (house mouse) ]
• Official Symbol: Il21
• Synonyms: IL21; interleukin 21; interleukin-21; IL-21
• Gene ID: 60505
• mRNA Refseq: NM_021782
• Protein Refseq: NP_068554
• UniProt ID: Q9ES17