Active Recombinant Mouse Il1b Protein

• Cat.No.: Il1b-102M
• Product Overview: Purified recombinant protein of Mouse interleukin 1 beta (Il1b) (Val118-Ser269) without tag was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 118-269 a.a.
• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response.
• Bio-activity: The ED50 is 0.001 -0.005 ng/ml, corresponding to a specific activity of 1- 0.2 x 10^9 units/mg, determined by the dose dependent stimulation of D10S cells proliferation
• Molecular Mass: 17.4 kDa
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Storage Buffer: 10mM NaH2PO4, 150mM NaCl, pH7.2

Gene Information

• Gene Name: Il1b interleukin 1 beta [ Mus musculus (house mouse) ]
• Official Symbol: Il1b
• Synonyms: Il1b; interleukin 1 beta; Il-1b; IL-1beta; interleukin-1 beta; IL-1 beta
• Gene ID: 16176
• mRNA Refseq: NM_008361
• Protein Refseq: NP_032387
• UniProt ID: P10749