Active Recombinant Mouse Il1b Protein

• Cat.No.: Il1b-101M
• Product Overview: Recombinant protein of mouse Interleukin-1B/IL-1B (Val118-Ser269) without tag was expressed in E.coli.

Specification

• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response.
• Source: E. coli
• Species: Mouse
• Bio-activity: ED50 is less than 0.01 ng/ml as determined by the dose-dependent stimulation of mouse D10S cells.
  Specific Activity of 1.0 x 108 IU/mg.
• Molecular Mass: 17.4 kDa
• Endotoxin: < 0.1 EU per µg protein as determined by LAL test
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a 0.2 µM filtered solution of 50mM Tris-HCl, 50mM NaCl, pH 8.0.
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.
• Protein length: 118-269 a.a.

Gene Information

• Gene Name: Il1b interleukin 1 beta [ Mus musculus (house mouse) ]
• Official Symbol: Il1b
• Synonyms: Il1b; interleukin 1 beta; Il-1b; IL-1beta; interleukin-1 beta; IL-1 beta
• Gene ID: 16176
• mRNA Refseq: NM_008361
• Protein Refseq: NP_032387
• UniProt ID: P10749