Recombinant Human IL1B, His-tagged

• Cat.No.: IL1B-321H
• Product Overview: IL1B Human Recombinant produced in E.Coli is a single, non-glycosylated, His-Tag Polypeptide chain containing 153 amino acids fragment (117-269) and having a total molecular mass of 21.88 kDa with an amino-terminal hexahistidine tag. The IL-1B His is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 117-269 a.a.
• Description: IL1B is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL1B binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL1B is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.
• Form: IL1B His is supplied in 1μ PBS and 50% glycerol.
• Research topic: Energy metabolism and body weight regulation
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

Gene Information

• Gene Name: IL1B interleukin 1, beta [ Homo sapiens ]
• Official Symbol: IL1B
• Synonyms: IL1B; interleukin 1, beta; IL-1; IL1F2; IL1-BETA; interleukin-1 beta; IL-1 beta; catabolin; OTTHUMP00000162031; preinterleukin 1 beta; pro-interleukin-1-beta
• Gene ID: 3553
• mRNA Refseq: NM_000576
• Protein Refseq: NP_000567
• MIM: 147720
• UniProt ID: P01584
• Chromosome Location: 2q14
• Pathway: African trypanosomiasis; Alzheimer"s disease; Amoebiasis; Apoptosis; Cellular roles of Anthrax toxin; Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; Graft-versus-host disease; Hematopoietic cell lineage; IFN-gamma pathway; IL-1 Signaling Pathway; IL12-mediated signaling events; IL27-mediated signaling events; IL23-mediated signaling events; Influenza A
• Function: cytokine activity; growth factor activity; interleukin-1 receptor binding; protein binding; protein domain specific binding