Active Recombinant Mouse IL12A & IL12B Heterotrimer Protein (Met1-Ala215 & Met1-Ser335), HlgG1 Fc-tagged

• Cat.No.: IL12-1031M
• Product Overview: Recombinant Mouse IL12A & IL12B Heterotrimer Protein (P43431)(Met1-Ala215) & (P43432)(Met1-Ser335) was produced by HEK293 Cells expression system. This protein was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid. The two plasmids were co-expressed and the heterodimer was purified.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ala215 & Met1-Ser335
• Predicted N Terminal: Arg 23 & Met 23
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized mouse mIL12A+mIL12B at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His, The EC50 of biotinylated human IL12RB1-His is 38.7-90.3 ng/ml.
  2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.2-0.8 ng/mL.
• Molecular Mass: The recombinant mouse IL12 heterodimer of IL12A/IL12B, both was Fc chimera, comprises 988 (434 + 554) amino acids and has a calculated molecular mass of 111.5 (48.7 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 75 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Il12a interleukin 12a [ Mus musculus ] & Il12b interleukin 12b [ Mus musculus ]
• Official Symbol: IL12A & IL12B
• Synonyms: IL12A; CLMF p35; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232 & IL12B; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; p40; Il-12b; Il12p40; Il-12p40
• Gene ID: 16159 & 16160
• mRNA Refseq: NM_001159424 & NM_008352
• Protein Refseq: NP_001152896 & NP_032378
• UniProt ID: P43431 & P43432