Active Recombinant Mouse interleukin 12 Protein, Flag tagged

• Cat.No.: Il12b-175M
• Product Overview: Recombinant mouse IL-12B protein (P43432) (Met 23-Ser 335) and mouse IL-12A protein (P43431) (Arg 23-Ala 215) linked by a GSGSSRGGSGSGGSGGGGSK linker with a Flag tag at the C-terminus was expressed in HEK293.

Specification

• Description: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
• Source: HEK293
• Species: Mouse
• Tag: C-Flag
• Protein length: IL-12B: 23-335aa
  IL-12A: 23-215aa
• Bio-activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 15-90 ng/mL.
• Molecular Mass: 60.6 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.
• GeneID 2: 16159

Gene Information

• Gene Name: Il12b interleukin 12b [ Mus musculus (house mouse) ]
• Official Symbol: Il12b
• Synonyms: IL12B; interleukin 12b; interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; p40; Il-12b; Il12p40; Il-12p40
• Gene ID: 16160
• mRNA Refseq: NM_008352
• Protein Refseq: NP_032378
• UniProt ID: P43432
• Gene Name 2: Il12a interleukin 12a [ Mus musculus (house mouse) ]
• Official Symbol 2: IL12A
• Synonyms 2: IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232
• mRNA Refseq 2: NM_001159424
• Protein Refseq 2: NP_001152896
• UniProt ID 2: P43431