Active Recombinant Mouse Icosl protein(Met1-Lys279), His&hFc-tagged

• Cat.No.: Icosl-594M
• Product Overview: Recombinant Mouse B7-H2 (NP_056605.1) extracellular domain (Met 1-Lys 279) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His&hFc
• Protein length: Met1-Lys279
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Mouse ICOS / AILIM / CD278 Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Mouse ICOS Ligand / B7-H2 / ICOSLG Protein (His & Fc Tag), The EC50 is 25-80 ng/mL.
• Molecular Mass: The secreted recombinant mouse B7-H2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 481 amino acids with a predicted molecular mass of 54.3 kDa. As a result of glycosylation, it migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Icosl icos ligand [ Mus musculus ]
• Official Symbol: Icosl
• Synonyms: ICOSL; icos ligand; ICOS ligand; B7 homolog 2; B7-like protein Gl50; B7-related protein 1; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653; KIAA0653
• Gene ID: 50723
• mRNA Refseq: NM_015790
• Protein Refseq: NP_056605