Active Recombinant Rabbit FCGRT&B2M protein, His-Avi-tagged, Biotinylated

• Cat.No.: FCGRT&B2M-1419R
• Product Overview: Biotinylated Recombinant Rabbit FCGRT&B2M protein(Ala24-Val297(FCGRT)&Val22-Tyr120(B2M)), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Rabbit
• Source: HEK293
• Tag: His&Avi
• ProteinLength: 24-297;22-120 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free captured on Biotin CAP-Series S Sensor Chip can bind Herceptin with an affinity constant of 1.05 μM as determined in a SPR assay (Biacore 8K).Immobilized Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free on SA Chip can bind Herceptin with an affinity constant of 1.53 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 33.8 kDa (FCGRT) & 11.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-40 kDa (FCGRT) & 12 kDa (B2M) respectively due to glycosylation.
• Proteinlength: Ala24-Val297(FCGRT)&Val22-Tyr120(B2M)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.