Active Recombinant Cynomolgus / Rhesus macaque FCGRT&B2M protein, His-Avi-tagged, Biotinylated

• Cat.No.: FCGRT&B2M-1423C
• Product Overview: Biotinylated Recombinant Cynomolgus / Rhesus macaque FCGRT&B2M protein(Ala24-Ser297(FCGRT)&Ile21-Met119(B2M)), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: Avi&His
• Protein Length: 24-297;21-119 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Loaded Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag on SA Biosensor, can bind Cynomolgus Serum Albumin, His Tag with an affinity constant of 0.617 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus , followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-40 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
• Proteinlength: Ala24-Ser297(FCGRT)&Ile21-Met119(B2M)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.