Active Recombinant Mouse F10 Protein, His-tagged

• Cat.No.: F10-1058M
• Product Overview: Recombinant Mouse F10 (Accession # O88947) Met1-Asn481, fused with a C-terminal 10-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Mouse
• Source: Sf21 Cells
• Tag: His
• Protein Length: Met1-Asn481
• Predicted N Terminal: Tyr84, Ile232
• Form: Supplied as a 0.2 µm filtered solution in MES, NaCl and CaCl2.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The specific activity is >300 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
• Molecular Mass: Recombinant Mouse F10, His-tagged has a calculated MW of 29 kDa & 10 kDa. In SDS-PAGE migrates as 30-38 kDa & 13-15 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: F10 coagulation factor X [ Mus musculus ]
• Official Symbol: F10
• Synonyms: F10; coagulation factor X; stuart factor; fX; Cf10
• Gene ID: 14058
• mRNA Refseq: NM_001242368
• Protein Refseq: NP_001229297