Recombinant Mouse F10 Protein, MYC/DDK-tagged

• Cat.No.: F10-993M
• Product Overview: Purified recombinant protein of full-length mouse coagulation factor X (F10), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Description: This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 54 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: F10 coagulation factor X [ Mus musculus (house mouse) ]
• Official Symbol: F10
• Synonyms: F10; coagulation factor X; fX; Cf10; coagulation factor X; stuart factor; EC 3.4.21.6
• Gene ID: 14058
• mRNA Refseq: NM_007972
• Protein Refseq: NP_031998
• UniProt ID: O88947