Active Recombinant Mouse Endothelial Cell-Specific Molecule 1, His-tagged

• Cat.No.: Esm1-476M
• Product Overview: RecombinantMouse Esm1 protein with a C-terminal10-His tag was expressed in Murinemyeloma cell line, NS0-derived. Trp20 - Arg184.

Specification

• Cat. No.: Esm1-476M
• Description: Esm1 (endothelialcell proteoglycan; also known as endothelialcell specific molecule1 [ESM1]), isa 50 kDa, monomeric, secreted, cysteinerich proteoglycan identified initiallyin endothelial cells of the kidney and lung. Mouse Esm1 is synthesized as a184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20kDa, 163 aa mature region. The Nterminal 2/3 of the molecule contains 18cysteine residues and there are no potential Nlinked glycosylation sites.Based on human Endocan, there are at least two potential Olinked glycosylationsites, one of which will likely be utilized on Ser at position 136 of themature molecule.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Trp20
• MolecularWeight: 19.4 kDa
• Activity: Measured by theability of the immobilized protein to support the adhesion of NIH/3T3 mouseembryonic fibroblasts. rmEndocan immobilized at 25 μg/mL, 100 μL/well, willmediate > 20% cell adhesion (100 μL/well at 0.5 x 106 cells/mL).
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >90%, by SDSPAGE underreducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 100μg/mL in sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
• Protein length: 20-184 a.a.

Gene Information

• Gene Name: Esm1 endothelial cell-specificmolecule 1 [ Mus musculus ]
• Official Symbol: Esm1
• Synonyms: Esm1; endothelialcell-specific molecule 1; ESM-1; AV004503; 0610042H23Rik; endothelialcell-specific molecule 1
• Gene ID: 71690
• mRNA Refseq: NM_023612
• Protein Refseq: NP_076101
• Chromosome Location: 13 D2.2; 13
• Function: insulin-like growthfactor binding; molecular_function