Active Recombinant Mouse Efnb2 protein, His-tagged

• Cat.No.: Efnb2-3315M
• Product Overview: Recombinant Mouse Efnb2 protein(NP_034241.2)(Met1-Ala232), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ala232
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized Ephrin B2 Protein, Mouse, Recombinant (His Tag) at 2 μg/mL (100 μL/well) can bind EphB4 Protein, Mouse, Recombinant (hFc Tag), the EC50 is 3-18 ng/mL.
• Molecular Mass: The secreted recombinant mouse EFNB2 comprises 215 amino acids and has a predicted molecular mass of 23.5 kDa. As a result of glycosylation, the apparent molecular mass of rmEFNB2 is approximately 30-40 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Efnb2 ephrin B2 [ Mus musculus ]
• Official Symbol: Efnb2
• Synonyms: EFNB2; ephrin B2; ephrin-B2; HTK ligand; EPH-related receptor tyrosine kinase ligand 5; Epl5; ELF-2; Eplg5; Htk-L; Lerk5; LERK-5; NLERK-1
• Gene ID: 13642
• mRNA Refseq: NM_010111
• Protein Refseq: NP_034241