Active Recombinant Human EFNB2 Protein, His-tagged

• Cat.No.: EFNB2-2032H
• Product Overview: Recombinant Human Ephrin-B2/EFNB2 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met 1-Ala 229) of human Ephrin-B2/EFNB2 (Accession #NP_004084.1) fused with a 6×His tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA . Immobilized human EFNB2 at 2 μg/ml (100 μl/well) can bind human EphB4 with a linear range of 1.56-12.5 ng/ml .
• Molecular Mass: The recombinant human EFNB2 consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EFNB2 is approximately 35-40 kDa due to glycosylation.
• Protein length: Met 1-Ala 229
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: EFNB2 ephrin-B2 [ Homo sapiens ]
• Official Symbol: EFNB2
• Synonyms: EFNB2; ephrin-B2; EPLG5; eph related receptor tyrosine kinase ligand 5; HTK ligand; Htk L; HTKL; LERK5; ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; LERK-5; ligand of eph-related kinase 5; eph-related receptor tyrosine kinase ligand 5; Htk-L
• Gene ID: 1948
• mRNA Refseq: NM_004093
• Protein Refseq: NP_004084
• MIM: 600527
• UniProt ID: P52799

Reviews

01/05/2022

During the experiment, the technician helped me solve the problems in a timely and accurate manner.

01/02/2022

The EFNB2 proteins purchased from your company is suitable for WB.

01/23/2021

Received high quality protein products and obtained satisfactory experimental results.

Q&As

What effect does EphB6 overexpression have on EFNB2?

EphB6 overexpression up-regulates the expression of its ligand EFNB2.

What diseases is EFNB2 involved in?

EFNB2 is associated with breast, colon, bladder, cervical and other cancers.

What is the biological function of EFNB2?

EFNB2 is involved in tissue plasticity, tumorigenesis, and other biological processes.

Where is EFNB2 mainly expressed?

EFNB2 is widely expressed in tumor vasculature.

What is the association between EFNB2 and BUC cells?

EFNB2 promotes BUC cell proliferation by inhibiting apoptosis in BUC cells.

How is EphB6 related to EFNB2?

EFNB2 is one of the physiological ligands of EphB6 that functions by binding to EphB6.