Active Recombinant Mouse EFNB2 Protein, Fc-tagged

• Cat.No.: EFNB2-882M
• Product Overview: Recombinant mouse EFNB2 (NP_034241.2) extracellular domain (Met 1-Ala 232) was expressed with the fused Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Arg 29
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EphB4 at 2 μg/ml (100 μl/well) can bind mouse EphrinB2 Fc with a linear range of 0.2-25 ng/ml.
• Molecular Mass: The recombinant mouse EFNB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 445 amino acids and has a predicted molecular mass of 49 kDa. As a result of glycosylation, the apparent molecular mass of rmEFNB2/Fc monomer is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 1-232 a.a.

Gene Information

• Gene Name: Efnb2 ephrin B2 [ Mus musculus ]
• Official Symbol: EFNB2
• Synonyms: EFNB2; ephrin B2; ephrin-B2; HTK ligand; EPH-related receptor tyrosine kinase ligand 5; Epl5; ELF-2; Eplg5; Htk-L; Lerk5; LERK-5; NLERK-1
• Gene ID: 13642
• mRNA Refseq: NM_010111
• Protein Refseq: NP_034241
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: ephrin receptor binding; protein binding; receptor binding