Active Recombinant Mouse Dner Protein, Fc Chimera

• Cat.No.: Dner-838M
• Product Overview: Recombinant Mouse Dner (Accession # NP_690879), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Ala26
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit Pleiotrophin-induced neurite outgrowth of E16-E18 rat embryonic cortical neurons.When 4 x 10^4 neurons/well are added to 96-well plate containing serial dilutions of Recombinant Mouse DNER Fc Chimera and immobilized Recombinant Mouse Pleiotrophin/PTN (1 µg/mL), neurite outgrowth is significantly inhibited in a dose dependent manner. The ED50 for this effect is typically 0.5 - 2.5 µg/mL.
• Molecular Mass: Recombinant Mouse Dner, Fc Chimera has a calculated MW of 91.4 kDa (reduced). In SDS-PAGE migrates as 140-160 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Dner delta/notch-like EGF-related receptor [ Mus musculus ]
• Official Symbol: Dner
• Synonyms: DNER; delta/notch-like EGF-related receptor; delta and Notch-like epidermal growth factor-related receptor; transmembrane protein Bet; brain EGF repeat-containing transmembrane protein; BET; Bret; A930026D19Rik; MGC39059
• Gene ID: 227325
• mRNA Refseq: NM_152915
• Protein Refseq: NP_690879