Active Recombinant Mouse Dlk1 protein, His-tagged

• Cat.No.: Dlk1-216M
• Product Overview: Recombinant Mouse Dlk1(Ala24-Gln305) fused with His tag at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ala24
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit adipocyte differentiation of mouse 3T3‑L1 mouse embryonic fibroblast adipose-like cells. Smas, C.M. et al. (1993) Cell 73:725. Recombinant Mouse Pref‑1/DLK1/FA1 at 2.5 μg/mL will inhibit adipocyte differentiation by more than 50%.
• Molecular Mass: Predicted Molecular Mass: 31 kDa;SDS-PAGE: 49-66 kDa, reducing conditions.
• Protein length: Ala24-Gln305
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: Dlk1 delta-like 1 homolog (Drosophila) [ Mus musculus ]
• Official Symbol: Dlk1
• Synonyms: DLK1; delta-like 1 homolog (Drosophila); protein delta homolog 1; fetal antigen 1; preadipocyte factor 1; stromal cell derived protein 1; adipocyte differentiation inhibitor protein; FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
• Gene ID: 13386
• mRNA Refseq: NM_001190703
• Protein Refseq: NP_001177632
• Chromosome Location: 12 60.17 cM; 12 E-F1
• Pathway: Adipogenesis, organism-specific biosystem
• Function: calcium ion binding