Active Recombinant Human DLK1, His-tagged

Cat.No. : DLK1-195H
Product Overview : Recombinant Human DLK1 (Accession # AAA75364) Ala24-Pro297 (Arg248Pro) (Lys295Ser), fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
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Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : Ala24 & Phe27
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured by its ability to inhibit adipocyte differentiation of mouse 3T3-L1 mouse embryonic fibroblast adipose-like cells. Smas, C.M. et al. (1993) Cell 73:725.3.3 ug/mL of Recombinant Human Pref-1/DLK-1/FA1 will inhibit adipocyte differentiation by more than 50%.
Molecular Mass : Recombinant Human DLK1, His-tagged has a calculated MW of 29.7 kDa. In SDS-PAGE migrates as 40-55 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 250 µg/mL in PBS.
Protein length : 24-297 a.a.
Publications :
The Special Role of JAK/STAT, and Notch Signaling Pathways in Cancer Pathogenesis (2023)
Gene Name DLK1 delta-like 1 homolog (Drosophila) [ Homo sapiens ]
Official Symbol DLK1
Synonyms DLK1; delta-like 1 homolog (Drosophila); delta like homolog (Drosophila); protein delta homolog 1; Delta1; FA1; pG2; Pref 1; ZOG; DLK-1; secredeltin; fetal antigen 1; preadipocyte factor 1; DLK; PREF1; Pref-1;
Gene ID 8788
mRNA Refseq NM_003836
Protein Refseq NP_003827
MIM 176290
UniProt ID P80370
Chromosome Location 14q32

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