Active Recombinant Mouse CTSS Protein, His-tagged

• Cat.No.: CTSS-911M
• Product Overview: Recombinant full length of mouse CTSS (AAB94925.1) (Met 1-Ile 340) was expressed, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: Val 18
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVENval-WRK (Dnp)-NH2. The specific activity is >300 pmoles/min/μg.
• Molecular Mass: The recombinant mouse CTSS consists of 334 amino acids and predicts a molecular mass of 37.6 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCTSS is approximately 33-38 KDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: Met1-Ile340

Gene Information

• Gene Name: Ctss cathepsin S [ Mus musculus ]
• Official Symbol: CTSS
• Synonyms: CTSS; cathepsin S; Cat S
• Gene ID: 13040
• mRNA Refseq: NM_021281
• Protein Refseq: NP_067256
• Pathway: Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Phagosome, organism-specific biosystem; Phagosome, conserved
• Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; hydrolase activity; peptidase activity

Reviews

11/01/2021

High catalytic efficiency, short time, saving time.

06/13/2021

The solubility is very good and can be easily dissolved in various buffers.

03/27/2021

Anti-pollution ability is very strong, not easy to be polluted.

Q&As

How can the function of CTSS proteins be studied by gene knockout or knockdown techniques?

The function of CTSS proteins can be studied through gene knockout or knockdown techniques, such as using techniques such as CRISPR-Cas9 or RNAi. These techniques can create cellular models of missing or reduced CTSS proteins to observe their effects on cell death, among other things.

How can I detect the expression level of CTSS protein?

Expression level of CTSS protein can be detected by immunohistochemistry, western blotting, and real-time quantitative PCR.

How is CTSS protein involved in the process of apoptosis?

CTSS proteins promote cell death by degrading proteins inside cells. During apoptosis, CTSS proteins degrade key proteins within cells, leading to cell death.

Are the interactions between CTSS proteins and other proteins regulated?

The interaction between CTSS proteins and other proteins is regulated by the formation of complexes or the regulation of signal transduction pathways. These interactions can be influenced by a variety of factors, such as ion concentrations, neurotransmitters, and growth factors.

What is the relationship between the expression level of CTSS protein and the occurrence and progression of diseases?

Some studies have shown that the expression level of CTSS protein may be associated with the onset and progression of certain diseases. For example, in some cancers, the expression level of the CTSS protein may change.

In which organelles is CTSS protein expressed?

It is mainly expressed in lysosomes, which is one of the major proteases in lysosomes.