Active Recombinant Mouse Collagen, Type XIII, Alpha 1, His-tagged

• Cat.No.: Col13a1-465M
• Product Overview: Recombinant Mouse Col13a1protein was expressed in Murine myeloma cell line with a N-terminal 6-His tag.Glu107-Gln565.

Specification

• Cat. No.: Col13a1-465M
• Description: Col13a1 is an 85 kDa95 kDa protein in the type 2 transmembrane collagen family. Mature mouse Col13a1consists of a 40 amino acid (aa) cytoplasmic domain, a 19 aa transmembrane segment,and a 692 aa extracellular domain (ECD). The ECD contains three collagenous regionsseparated by shorter noncollagenous regions. Within comparable regions of theECD, mouse Col13a1 shares 85% and 88% aa sequence identity with human and ratCol13a1, respectively.
• Source: Mammalian cells
• Form: Lyophilized from a 0.2μm filtered solution in PBS.
• N-terminalSequence: His
• Molecular Weight: 46.0 kDa
• Activity: Measuredby the ability of the immobilized protein to support the adhesion of A431 humanepidermoid carcinoma cells. When 1 x 105 cells per well are added tormCol13a1 coated plate (2 μg/mL, 100 μL/well), approximately 45%-75% will adhereafter 1 hour at 37°C. Optimal dilutions should be determined by each laboratoryfor each application.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• Purity: >80%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 300 μg/mL in PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
• Protein length: 107-565 a.a.

Gene Information

• Gene Name: Col13a1 collagen,type XIII, alpha 1 [ Mus musculus ]
• Official Symbol: Col13a1
• Synonyms: Col13a1; collagen,type XIII, alpha 1; collagen alpha-1(XIII) chain; type XIII collagen; procollagen,type XIII, alpha 1
• Gene ID: 12817
• mRNA Refseq: NM_007731
• Protein Refseq: NP_031757
• UniProt ID: Q9R1N9
• Chromosome Location: 10 B4; 10 31.5 cM
• Function: heparin binding