Active Recombinant Mouse Col13a1, His-tagged

• Cat.No.: Col13a1-325M
• Product Overview: Recombinant Mouse Col13a1 (Accession # AAH34164) Glu107-Gln565, fused with an N-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.When 1 x 10^5 cells per well are added to rmCOL-13A1 coated plate (2 µg/mL, 100 µL/well), approximately 45% - 75% will adhere after 1 hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Col13a1, His-tagged has a calculated MW of 46.0 kDa. In SDS-PAGE migrates as 50-60 kDa, reducing conditions.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 107-565 a.a.

Gene Information

• Gene Name: Col13a1 collagen, type XIII, alpha 1 [ Mus musculus ]
• Official Symbol: Col13a1
• Synonyms: COL13A1; collagen, type XIII, alpha 1; collagen alpha-1(XIII) chain; type XIII collagen; procollagen, type XIII, alpha 1
• Gene ID: 12817
• mRNA Refseq: NM_007731
• Protein Refseq: NP_031757