Active Recombinant Mouse Cd74 Protein, HA-tagged

• Cat.No.: Cd74-641M
• Product Overview: Recombinant Mouse Cd74 (Accession # NP_034675) Gln56-Leu215, fused with an N-terminal HA-tag (YPYDVPDYA), was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Tag: HA
• Predicted N Terminal: Tyr
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.When 5 x 10^4 cells per well are added to Recombinant Mouse CD74 coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 0.3-1.5 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Cd74, HA-tagged has a calculated MW of 19.4 kDa. In SDS-PAGE migrates as 26-40 kDa, reducing conditions.
• Protein length: Gln56-Leu215
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Cd74 CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) [ Mus musculus ]
• Official Symbol: Cd74
• Synonyms: CD74; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); H-2 class II histocompatibility antigen gamma chain; HLA-DR-GAMMA; dinucleotide microsatellite; Ia-associated invariant chain; ia antigen-associated invariant chain; MHC class II-associated invariant chain; class II-associated invariant chain peptide; histocompatibility: class II antigens, gamma chain of; invariant polypeptide of major histocompatibility complex, class II antigen-associated; Ii; CLIP; DHLAG; HLADG; Ia-GAMMA
• Gene ID: 16149
• mRNA Refseq: NM_001042605
• Protein Refseq: NP_001036070