Active Recombinant Human CD74, HA-tagged

• Cat.No.: CD74-2232H
• Product Overview: Recombinant Human CD74 (Gln56-Leu215), fused with an HA tag at N-terminal, was expressed in CHO Cells.

Specification

• Cat. No.: CD74-2232H
• Description: The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: Chinese Hamster Ovary cell
• N-terminalSequenceAnalysis: Tyr
• PredictedMolecularMass: 19.4 kDa
• SDS-PAGE: 26-40kDa, reducing conditions
• Activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.When 5 x 104 cells per well are added to Recombinant Mouse CD74 coated plates, cell adhesion is enhanced in a dose dependent manner. TheED50 for this effect is typically 0.3-1.5μg/mL.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDSPAGEunder reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Stability &Storage: Use a manual defrost freezer and avoid repeated freezethawcycles.12 months from date of receipt, 20to 70°C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, 20to 70°C under sterile conditions after reconstitution.
• Protein length: 56-215 a.a.

Gene Information

• Gene Name: CD74 CD74 molecule, major histocompatibility complex, class II invariant chain [ Homo sapiens ]
• Official Symbol: CD74
• Synonyms: CD74 molecule, major histocompatibility complex, class II invariant chain; DHLAG; HLADG; Ia-GAMMA; CD74; HLA class II histocompatibility antigen gamma chain; ii; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; ia antigen-associated invariant chain; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 antigen
• Gene ID: 972
• mRNA Refseq: NM_004355
• Protein Refseq: NP_004346
• MIM: 142790
• UniProt ID: P04233
• Chromosome Location: 5q32
• Pathway: Antigen processing and presentation
• Function: MHC class II protein binding; beta-amyloid binding; cytokine binding; cytokine receptor activity; identical protein binding