Active Recombinant Mouse C2 Protein, His-tagged

• Cat.No.: C2-1012M
• Product Overview: Recombinant Mouse C2 (Accession # O70350) Met1-Leu760, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Leu20 & Lys251
• Form: Supplied as a 0.2 µm filtered solution in Sodium Acetate and NaCl.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5"Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >200 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse C2, His-tagged has a calculated MW of 58 kDa and 84 kDa. In SDS-PAGE migrates as 66-75 kDa & 90-110 kDa, reducing conditions.
• Protein length: Met1-Leu760
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: C2 complement component 2 (within H-2S) [ Mus musculus ]
• Official Symbol: C2
• Synonyms: C2; complement component 2 (within H-2S); complement C2; C3/C5 convertase; complement factor C2; MHC complement component C2; classical-complement pathway C3/C5 convertase
• Gene ID: 12263
• mRNA Refseq: NM_013484
• Protein Refseq: NP_038512