Active Recombinant Mouse Anxa2 Protein, His-tagged

• Cat.No.: Anxa2-3494M
• Product Overview: Active Recombinant Mouse Anxa2 Protein, His-tagged,expressed in E. coli.

Specification

• Description: Annexin A2 (ANXA2) is a calcium-regulated membrane-binding protein with heightened calcium affinity in the presence of anionic phospholipids. Known for its potential involvement in the heat-stress response, ANXA2 operates as a heterotetramer, comprising two light chains of S100A10/p11 and two heavy chains of ANXA2/p36. It inhibits PCSK9-enhanced LDLR degradation, potentially through translational mechanisms, while also competing with LDLR for PCSK9 binding. ANXA2 interacts with various proteins, including ATP1B1, DYSF, COCH, and CEACAM1, with the latter interaction being regulated by CEACAM1 phosphorylation. Moreover, ANXA2 interacts with PCSK9, forming a complex that hinders LDLR degradation. Additionally, it associates with APPL2 and APPL1, facilitating the targeting of APPL2 to endosomes in parallel with RAB5A. The intricate network of ANXA2 interactions underscores its versatile role in cellular processes and regulatory pathways. Annexin A2/ANXA2 Protein, Mouse (His) is the recombinant mouse-derived Annexin A2/ANXA2 protein, expressed by E. coli , with N-His labeled tag. The total length of Annexin A2/ANXA2 Protein, Mouse (His) is 338 a.a., with molecular weight of ~39.6 kDa.
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Protein length: 2-339 aa
• Form: Lyophilized powder
• Bio-activity: Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 0.1183 ng/mL, corresponding to a specific activity is 8.453×106 units/mg.
• Molecular Mass: 39.6 kDa
• AA Sequence: STVHEILCKLSLEGDHSTPPSAYGSVKPYTNFDAERDALNIETAVKTKGVDEVTIVNILTNRSNVQRQDIAFAYQRRTKKELPSALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARELYDAGVKRKGTDVPKWISIMTERSVCHLQKVFERYKSYSPYDMLESIKKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
• Purity: > 95% as determined by reducing SDS-PAGE
• Storage: Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, 4 mM DTT, pH 7.4 or 20 mM PBS, 150 mM NaCl, pH 8.0.
• Shipping: Room temperature in continental US; may vary elsewhere.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Gene Information

• Gene Name: Anxa2 annexin A2 [ Mus musculus (house mouse) ]
• Official Symbol: Anxa2
• Synonyms: Chromobindin-8; Lipocortin II; PAP-IV; ANX2; ANX2L4; CAL1H; LPC2D; ANXA2; Annexin A2; Annexin II; LIP2PAP-IV; LPC2D; LPC2DP36; p36; Anxa2
• Gene ID: 12306
• UniProt ID: Q542G9