Active Recombinant Mouse Alkaline Phosphatase, Liver/Bone/Kidney, His-tagged

• Cat.No.: Alpl-444M
• Product Overview: Recombinant Mouse Alplprotein was expressed in Murine myeloma cell line. Phe18-Gly503, witha C terminal 6 His tag.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 18-503 a.a.
• Description: Severaldistinct genes encode alkaline phosphatases (APs) in mice with differenttissuespecific expression patterns. The Alpl gene, also known as Akp2,encodes the liver/bone/kidney isozyme, also known as the tissuenonspecific AP(TNAP). The Alpl gene is a key regulator of bone mineralization in mice. Avariety of mutations in the human Alpl gene leads todifferent forms of hypophosphatasia, characterized by poorly mineralizedcartilage and bones.
• Form: Suppliedas a 0.2 um filtered solution in Tris and NaCl.
• N-terminalSequence: Phe18
• MolecularWeight: 54 kDa
• Activity: Measuredby its ability to cleave a fluorogenic substrate, 4Methylumbelliferyl phosphate(4MUP). The specific activity is > 40000 pmoles/min/μg, as measured underthe described conditions. See Activity Assay Protocol.
• Endotoxin Level: <1.0EU per 1 μg of the protein by the LAL method.
• Purity: >95%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Storage: Do notfreeze. 3months from date of receipt, 2 to 8°C as supplied. 1 month, 2 to 8°C understerile conditions after opening.

Gene Information

• Gene Name: Alpl alkaline phosphatase,liver/bone/kidney [ Mus musculus ]
• Official Symbol: Alpl
• Synonyms: Alpl; alkalinephosphatase, liver/bone/kidney; Akp2; TNAP; Akp-2; APTNAP; TNSALP; alkalinephosphatase, tissue-nonspecific isozyme; alkaline phosphatase 2, liver; EC 3.1.3.1
• Gene ID: 11647
• mRNA Refseq: NM_007431
• Protein Refseq: NP_031457
• UniProt ID: P09242
• Chromosome Location: 4
• Pathway: Endochondral Ossification;Folate biosynthesis; Metabolic pathways; TNF-alpha NF-kB Signaling Pathway
• Function: alkaline phosphataseactivity; catalytic activity; hydrolase activity; metal ion binding; phosphataseactivity