Active Recombinant Mouse Adk Protein, His-tagged, Bioactivity Validated

• Cat.No.: Adk-245H
• Product Overview: Recombinant Mouse Adk(1-361aa) fused with His tag at N-terminal was expressed in E. coli. Bioactivity Validated.

Specification

• Description: Adk, also known as Adenosine kinase, is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid. In 20mM Tris-Hcl buffer (pH8.0) containing 20% glycerol, 50mM NaCl, 1mM EDTA
• Bio-activity: Specific activity is > 100 pmol/min/ and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.
• Molecular Mass: 42.5 kDa (384aa) confirmed by MALDI-TOF
• Protein length: 1-361aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAADEP KPKKLKVEAP QALSENVLFG MGNPLLDISA VVDKDFLDKY SLKPNDQILA EDKHKELFDE
  LVKKFKVEYH AGGSTQNSMK VAQWLIQEPH KAATFFGCIG IDKFGEILKR KAADAHVDAH YYEQNEQPTG TCAACITGGN RSLVANLAAA
  NCYKKEKHLD LERNWVLVEK ARVYYIAGFF LTVSPESVLK VARYAAENNR VFTLNLSAPF ISQFFKEALM DVMPYVDILF GNETEAATFA
  REQGFETKDI KEIAKKAQAL PKVNSKRQRT VIFTQGRDDT IVAAENDVTA FPVLDQNQEE IIDTNGAGDA FVGGFLSQLV SDKPLTECIR
  AGHYAASVII RRTGCTFPEK PDFH
• Purity: > 95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Adk adenosine kinase [ Mus musculus ]
• Official Symbol: Adk
• Synonyms: ADK; adenosine kinase; adenosine 5-phosphotransferase; Ak; AI255373; AI987814; 2310026J05Rik; 5033405D03Rik
• Gene ID: 11534
• mRNA Refseq: NM_134079
• Protein Refseq: NP_598840
• UniProt ID: P55264