Active Recombinant Human WNT3A Surrogate Protein, Fc-tagged, For Organoid Culture

• Cat.No.: WNT3A-186H
• Product Overview: Active Recombinant Human WNT3A Surrogate Protein, fused with C-terminal Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-Fc
• Form: Lyophilized from sterile PBS, pH 7.4, 3% trehalose.
• Bio-activity: Measured by its ability to induce Topflash reporter activity in HEK293 reporter cells. The ED50 for this effect is 10-50 ng/mL.
• Molecular Mass: The recombinant human WNT3A Surrogate Protein has a predicted molecular mass of 59.2 kDa (monomer). It migrates as an approximately 63-70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions.
• Storage: Store it under sterile conditions at -20°C to -80°C. Reconstituted protein stable at -80°C for 12 months, 4°C for 1 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Organoid Culture: 50-100ng/ml
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: WNT3A wingless-type MMTV integration site family, member 3A [ Homo sapiens ]
• Official Symbol: WNT3A
• Synonyms: WNT3A; wingless-type MMTV integration site family, member 3A; protein Wnt-3a; MGC119418; MGC119419; MGC119420
• Gene ID: 89780
• mRNA Refseq: NM_033131
• Protein Refseq: NP_149122
• MIM: 606359
• UniProt ID: P56704