Active Recombinant Human VEGFA, Met-tagged

• Cat.No.: VEGFA-425H
• Product Overview: Recombinant Human VEGFA (Accession # ABB58912) Ala27-Arg137, fused with an N-terminal Met & Pro28-Arg137, was produced in E. coli-derived.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 27-137;28-137 a.a.
• Predicted N Terminal: Met & Pro28
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED50 for this effect is typically 0.6-3 ng/mL
• Molecular Mass: Recombinant Human VEGFA, Met-tagged has a calculated MW of 13 kDa. In SDS-PAGE migrates as 12-13 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 ug/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• UniProt ID: P15692
• Chromosome Location: 6p12