Active Recombinant Human ULBP1 protein(Met1-Gly216), His&hFc-tagged

• Cat.No.: ULBP1-3249H
• Product Overview: Recombinant Human ULBP1 (NP_079494.1) (Met 1-Gly 216) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His&hFc
• Protein length: Met1-Gly216
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized human His-NKG2D (78-216) at 2 μg/ml (100 μl/well) can bind human ULBP1-Fch, The EC50 of human ULBP1-Fch is 8-24 ng/mL.
• Molecular Mass: The mature form of recombinant human ULBP1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 439 amino acids and predicts a molecular mass of 50.4 kDa. The apparent molecular mass of rhULBP1/Fc monomer is approximately 55-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ULBP1 UL16 binding protein 1 [ Homo sapiens ]
• Official Symbol: ULBP1
• Synonyms: ULBP1; UL16 binding protein 1; NKG2D ligand 1; RAET1I; N2DL-1; NKG2DL1; alcan-beta; UL16-binding protein 1; retinoic acid early transcript 1I
• Gene ID: 80329
• mRNA Refseq: NM_025218
• Protein Refseq: NP_079494
• MIM: 605697
• UniProt ID: Q9BZM6