Active Recombinant Human TNF protein, His-Avi-tagged, Biotinylated

• Cat.No.: TNF-361H
• Product Overview: Biotinylated Recombinant Human TNF protein(Val77-Leu233) is expressed from HEK293 with His tag and Avi tag at the C-terminal.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Val77-Leu233
• Form: Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TNF R2, mFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
• Molecular Mass: The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-24 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per ug by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375