Recombinant Human TNF protein, His-tagged

• Cat.No.: TNF-35H
• Product Overview: Recombinant human TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: TNF, also known as tumor necrosis factor, is cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 18.1kDa (163aa)18-28kDa (SDS-PAGE under reducing conditions)
• AA Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTKVNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >90% by SDS – PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375
• Chromosome Location: 6p21.3
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem
• Function: cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding