Active Recombinant Human THPO Protein (Ser22-Leu195), GMP Grade, Animal-Free

• Cat.No.: THPO-12HG
• Product Overview: GMP recombinant human Thrombopoietin protein (Ser22-Leu195) without tag was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Ser22-Leu195
• Description: Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
• Bio-activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.05-0.5 ng/mL.
  The specific activity of Recombinant Human Thrombopoietin is >1 x 107 units/mg, which is calibrated against the human Thrombopoietin reference standard.
• Molecular Mass: Predicted Molecular Mass: 18.7 kDa
  SDS-PAGE: 19 kDa, reducing conditions
• N-terminal Sequence Analysis: Ala-Ser22-Pro-Ala-Pro-Pro-Ala-(Cys)-Asp-Leu
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 6 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Acetate.
• Reconstitution: Reconstitute at 100-200 μg/mL in sterile, deionized water.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: THPO thrombopoietin [ Homo sapiens (human) ]
• Official Symbol: THPO
• Synonyms: THPO; thrombopoietin; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• Gene ID: 7066
• mRNA Refseq: NM_000460
• Protein Refseq: NP_000451
• MIM: 600044
• UniProt ID: P40225