Active Recombinant Human THPO protein, His-tagged, Biotinylated

• Cat.No.: THPO-216HB
• Product Overview: Recombinant Human THPO protein mature form (NP_000451.1) (Ser22-Gly353), fused with a polyhistidine tag at the N-terminus, was expressed in HEK293 Cells. The purified protein was biotinylated in vitro.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 22-353 a.a.
• Predicted N Terminal: Ser 22
• Form: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.
• Bio-activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 10-60 ng/ml.
• Molecular Mass: The recombinant human THPO consists of 348 amino acids and predicts a molecular mass of 37.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Official Symbol: THPO
• Synonyms: THPO; MGDF; MKCSF; ML; MPLLG; THCYT1; TPO
• Protein Refseq: NP_000451.1