Active Recombinant Human SULT1E1 Protein

• Cat.No.: SULT1E1-91H
• Product Overview: Recombinant human SULT1E1 protein without tag were coexpressed in E. coli.

Specification

• Description: Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors.
• Source: E. coli
• Species: Human
• Bio-activity: SULT Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride, 10 mM dithiothreitol and 20 μM PAPS)
  Substrate: 17β-estradiol (3-sulfate formation)
  Linear with time up to: 12 min(Determined at 20 nM substrate, using a protein concentration of 25 ng/ml)
  Linear with protein up to: 40 ng/ml(Determined at 20 nM substrate, using an incubation time of 5 min)
  Vmax: 22 nmol/min/mg protein
  Km: 5.0 nM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: Protein Concentration: 10 mg/ml
• Storage Buffer: 10 mM sodium phosphate (pH 7.4), 140 mM NaCl

Gene Information

• Gene Name: SULT1E1 sulfotransferase family 1E member 1 [ Homo sapiens (human) ]
• Official Symbol: SULT1E1
• Synonyms: SULT1E1; sulfotransferase family 1E member 1; EST; STE; EST-1; ST1E1; sulfotransferase 1E1; estrogen sulfotransferase; estrone sulfotransferase; sulfotransferase family 1E, estrogen-preferring, member 1; sulfotransferase, estrogen-preferring
• Gene ID: 6783
• mRNA Refseq: NM_005420
• Protein Refseq: NP_005411
• MIM: 600043
• UniProt ID: P49888