Active Recombinant Human SPARC Protein, His-tagged

• Cat.No.: SPARC-525H
• Product Overview: Recombinant Human SPARC (Accession # NP_003109) Ala18-Ile303, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Ala18-Ile303
• Predicted N Terminal: Ala18
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann, B.J. et al. (2003) Mol. Biol. Cell. 14:3977. The ED50 for this effect is typically 0.75 - 3.0 µg/mL.Measured by its binding ability in a functional ELISA.Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind rhSPARC with an apparent KD < 40 nm. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human SPARC, His-tagged has a calculated MW of 34 kDa. In SDS-PAGE migrates as 40-50 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SPARC secreted protein, acidic, cysteine-rich (osteonectin) [ Homo sapiens ]
• Official Symbol: SPARC
• Synonyms: SPARC; secreted protein, acidic, cysteine-rich (osteonectin); ON; cysteine rich protein; osteonectin; BM-40; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
• Gene ID: 6678
• mRNA Refseq: NM_003118
• Protein Refseq: NP_003109
• MIM: 182120
• UniProt ID: P09486
• Chromosome Location: 5q31-q33