Recombinant Human SPARC Protein, MBP-tagged

• Cat.No.: SPARC-001H
• Product Overview: Recombinant Human SPARC Protein (1-303 aa (full-length)) with MBP tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: MBP
• Protein Length: 1-303
• Description: This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 79.3 kDa
• Purity: >90% confirmed by 10% SDS-PAGE stained with Coomassie Blue (CB).
• Applications: ELISA, Western Blot, Antibody Production, Protein array
• Quality Control Test: 10% SDS-PAGE stained with Coomassie Blue (CB) immunoblotting with anti-MBP (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry.
• Storage: Store working aliquots at 4 centigrade for up to one week. For extended storage, conserve at −80 centigrade.
• Expiry: 12 months from the date of purchase, if it is properly stored.
• Storage Buffer: 20mM Tris-HCl, 0.2M NaCl, 1mM EDTA, 10mM Maltose, pH 7.4. No preservatives added.
• Shipping: Dry Ice

Gene Information

• Gene Name: SPARC secreted protein acidic and cysteine rich [ Homo sapiens (human) ]
• Official Symbol: SPARC
• Synonyms: SPARC; secreted protein acidic and cysteine rich; ON; ONT; OI17; BM-40; SPARC; basement-membrane protein 40; secreted protein, acidic, cysteine-rich (osteonectin)
• Gene ID: 6678
• mRNA Refseq: NM_003118
• Protein Refseq: NP_003109
• MIM: 182120
• UniProt ID: P09486