Active Recombinant Mouse Sparc, His-tagged

• Cat.No.: Sparc-4030M
• Product Overview: Recombinant Mouse Sparc (Ala18-Ile302) fused with 10­His tag at C­terminal, was expressed in Mouse NSO Cells.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 18-302 a.a.
• Description: SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM­40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N­terminal acidic region that binds calcium, a follistatin domain containing Kazal­like sequences, and a C­terminal extracellular calcium (EC) binding domain with two EF­hand motifs. Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N­glycosylation site are opposite this face. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC shows context­specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis. SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper­binding sequence KGHK. Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation. Stabilin­1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation. Mature mouse SPARC shows 97%, 92%, 92%, 92% and 83% aa identity with rat, human, dog, cow and chick SPARC, respectively.
• Predicted N Terminal: Ala18
• Formulation: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. The ED50 for this effect is typically 0.6 - 2.4 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: 34 kDa
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.

Gene Information

• Gene Name: Sparc secreted acidic cysteine rich glycoprotein [ Mus musculus(house mouse) ]
• Official Symbol: Sparc
• Synonyms: Sparc; secreted acidic cysteine rich glycoprotein; BM-40; AA517111; SPARC; ON; osteonectin; basement-membrane protein 40; secreted protein acidic and rich in cysteine
• Gene ID: 20692
• mRNA Refseq: NM_009242
• Protein Refseq: NP_033268
• UniProt ID: P07214
• Chromosome Location: 11 B1; 11 33.04 cM
• Pathway: Hemostasis; Platelet activation, signaling and aggregation; Platelet degranulation; Senescence and Autophagy
• Function: calcium ion binding; extracellular matrix binding; metal ion binding