Active Recombinant Human SIGLEC15 protein, Fc-tagged

• Cat.No.: SIGLEC15-15H
• Product Overview: Recombinant Human SIGLEC15(Phe20-Thr263) fused with Fc of Human IgG1 tag at C-terminal was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• ProteinLength: Phe20-Thr263
• Description: Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10. It is expressed on osteoclasts, macrophages, and dendritic cells and binds to the sialyl-Tn antigen. This interaction induces the production of TGF-beta by tumor-associated macrophages. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.
• Predicted N Terminal: Phe20
• Form: Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera binds with an ED50 of 150-900 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 53 kDa;
  SDS-PAGE: 53-64 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in water.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: SIGLEC15 sialic acid binding Ig like lectin 15 [ Homo sapiens ]
• Official Symbol: SIGLEC15
• Synonyms: CD33L3; HsT1361; SIGLEC-15; CD33 antigen-like 3; CD33 molecule-like 3
• Gene ID: 284266
• mRNA Refseq: NM_213602
• Protein Refseq: NP_998767
• UniProt ID: Q6ZMC9
• Chromosome Location: 18q12.3
• Pathway: DAP12 interactions, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem