Active Recombinant Human RSPO3 protein, Fc-tagged

• Cat.No.: RSPO3-0654H
• Product Overview: Recombinant Human RSPO3 protein(Gln22-His272), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 22-272 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human R-Spondin 3 Protein, Fc Tag at 5 μg/mL (100 μL/well) can bind Human LGR4, His Tag with a linear range of 0.02-0.625 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 54.9 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln22-His272
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RSPO3 R-spondin 3 [ Homo sapiens ]
• Official Symbol: RSPO3
• Synonyms: RSPO3; R-spondin 3; R spondin 3 homolog (Xenopus laevis) , thrombospondin, type I, domain containing 2 , THSD2; R-spondin-3; FLJ14440; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1
• Gene ID: 84870
• mRNA Refseq: NM_032784
• Protein Refseq: NP_116173
• MIM: 610574
• UniProt ID: Q9BXY4