Recombinant Human RSPO3 protein, For Organoid Culture

• Cat.No.: RSPO3-2866H
• Product Overview: Recombinant Human RSPO3 protein(Q9BXY4)(Gln22-His272) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Non
• Protein length: Gln22-His272
• Form: Lyophilized from sterile PBS, pH 7.4, 3% trehalose.
• Bio-activity: Measured by its ability to induce Topflash reporter activity in HEK293 reporter cells. The ED50 for this effect is 0.5-5.0ng/mL in the presence of 20 ng/mL Recombinant Wnt Surrogate Fc Chimera Protein.
• Molecular Mass: 28.3 kDa (monomer)
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions.
• Storage: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below: To the date of expiration, -20°C to -80°C as supplied. 3 months, -20°C to -80°C under sterile conditions after reconstitution. 1 month, 2 to 8 °C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RSPO3 R-spondin 3 [ Homo sapiens ]
• Official Symbol: RSPO3
• Synonyms: RSPO3; R-spondin 3; R spondin 3 homolog (Xenopus laevis) , thrombospondin, type I, domain containing 2 , THSD2; R-spondin-3; FLJ14440; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1
• Gene ID: 84870
• mRNA Refseq: NM_032784
• Protein Refseq: NP_116173
• MIM: 610574
• UniProt ID: Q9BXY4