Active Recombinant Human RNASE1 Protein, His-tagged

• Cat.No.: RNASE1-12H
• Product Overview: Recombinant human RNASE1 (29-156aa), fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 29-156 a.a.
• Description: This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
• Form: Liquid
• Bio-activity: Specific activity is > 3 X 10^6 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of RNA per minute at 25 centigrade.
• Molecular Mass: 15.3 kDa
• Identity: 134aa
• AA Sequence: KESRAKKFQRQHMDSDSSPSSSSTYCNQMMRRRNMTQGRCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYKSNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST
• N-terminal Sequence Analysis: Lys-Glu-Ser-Arg-Ala
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: RNASE1 ribonuclease A family member 1, pancreatic [ Homo sapiens (human) ]
• Official Symbol: RNASE1
• Synonyms: RNASE1; ribonuclease A family member 1, pancreatic; RAC1; RIB1; RNS1; ribonuclease pancreatic; HP-RNase; RIB-1; RNase 1; RNase A; RNase upI-1; ribonuclease 1; ribonuclease A C1; ribonuclease, RNase A family, 1 (pancreatic); EC 4.6.1.18
• Gene ID: 6035
• mRNA Refseq: NM_198235
• Protein Refseq: NP_937878
• MIM: 180440
• UniProt ID: P07998