Active Recombinant Human RET protein, His&GST-tagged

• Cat.No.: RET-2214H
• Product Overview: Recombinant Human RET protein(P07949-1)(His658-Ser1114), fused with His&GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: His658-Ser1114
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0.
• Bio-activity: The specific activity was determined to be 17 nmol/min/mg using synthetic TRK-C-derived Peptide (R11-VYSTDYYRLFNPS) as substrate.
• Molecular Mass: The recombinant human RET (aa 658-1114)/GST chimera consists of 694 amino acids and has a calculated molecular mass of 76.7 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RET ret proto-oncogene [ Homo sapiens ]
• Official Symbol: RET
• Synonyms: RET; ret proto-oncogene; Hirschsprung disease 1 , HSCR1, MEN2A, MEN2B, MTC1, multiple endocrine neoplasia and medullary thyroid carcinoma 1; proto-oncogene tyrosine-protein kinase receptor Ret; cadherin related family member 16; CDHF12; CDHR16; PTC; RET51; proto-oncogene c-Ret; receptor tyrosine kinase; RET transforming sequence; cadherin family member 12; hydroxyaryl-protein kinase; cadherin-related family member 16; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease); MTC1; HSCR1; MEN2A; MEN2B; RET-ELE1
• Gene ID: 5979
• mRNA Refseq: NM_020630
• Protein Refseq: NP_065681
• UniProt ID: P07949