Recombinant Human Ret Proto-Oncogene, GST-tagged

• Cat.No.: RET-5475H
• Product Overview: Recombinant humanRET catalytic domain (amino acids 658-1114) with GST tag, activated in vitrovia auto-phosphorylation.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 658-1114 a.a.
• Description: The RETproto-oncogene encodes a receptor tyrosine kinase for members of the glialcell line-derived neurotrophic factor family of extracellular signallingmolecules. RET loss of function mutations are associated with the developmentof Hirschsprung" s disease, while gain of function mutations are associatedwith the development of various types of human cancer.
• Form: Liquid in 50 mMTris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mM DTT +50% glycerol.
• Purity: ≥ 90% as determinedby SDS-PAGE analysis
• MolecularWeight: 79.0 kDa
• Specific Activity: 2382 nmole ofphosphate transferred to Abl1 peptide substrate (EAIYAAPFAKKK) per minute permg of total protein at 30°C. Activity determined at a final proteinconcentration of 1.67 μg/ml.
• Concentration: 0.31 mg/ml
• Storage: Stable for 6 monthsin working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: RET
• Pathways: Endocytosis;Pathways in cancer; SIDS Susceptibility Pathways; Signaling events regulatedby Ret tyrosine kinase; Thyroid cancer

Gene Information

• Gene Name: RET ret proto-oncogene [ Homosapiens ]
• Synonyms: RET; retproto-oncogene; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16;RET-ELE1; proto-oncogenetyrosine-protein kinase receptor Ret; OTTHUMP00000019479; OTTHUMP00000216967;proto-oncogene c-Ret; receptor tyrosine kinase; RET transforming sequence;cadherin family member 12; hydroxyaryl-protein kinase; cadherin-relatedfamily member 16; ret proto-oncogene (multiple endocrine neoplasia andmedullary thyroid carcinoma 1, Hirschsprung disease); EC 2.7.10.1; Proto-oncogene tyrosine-protein kinasereceptor Ret; Cadherinfamily member 12; Proto-oncogene c-Ret
• Gene ID: 5979
• mRNA Refseq: NM_020630
• Protein Refseq: NP_065681
• MIM: 164761
• UniProt ID: P07949
• Chromosome Location: 10q11.2
• Function: ATP binding; calciumion binding; nucleotide binding; protein binding; protein tyrosine kinaseactivity; receptor activity; transmembrane receptor protein tyrosine kinaseactivity