Active Recombinant Human PVRL1, His-tagged

• Cat.No.: PVRL1-3136H
• Product Overview: Recombinant Human PVRL1 (NP_002846.3) extracellular domain (Met 1-Thr 334), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Thr 334
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated recombinant human PVRL1 / Nectin-1 with a linear range of 6.4-800 ng/ml.
  2. Immobilized Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/ml (100 μl/well) can bind PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag), the EC50 of PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag)is 150-700 ng/mL.
• Molecular Mass: The recombinant human PVRL1 consists of 315 amino acids and predictes a molecular mass of 35.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPVRL1 is approximately 45-50 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PVRL1 poliovirus receptor-related 1 (herpesvirus entry mediator C) [ Homo sapiens ]
• Official Symbol: PVRL1
• Synonyms: PVRL1; poliovirus receptor-related 1 (herpesvirus entry mediator C); ED4, HVEC; poliovirus receptor-related protein 1; CD111; CLPED1; HIgR; nectin; OFC7; PRR; PRR1; PVRR1; SK 12; nectin 1; poliovirus receptor-like 1; herpesvirus Ig-like receptor; herpes virus entry mediator C; ectodermal dysplasia 4 (Margarita Island type); ED4; HVEC; PVRR; SK-12; nectin-1; MGC16207; MGC142031
• Gene ID: 5818
• mRNA Refseq: NM_002855
• Protein Refseq: NP_002846
• MIM: 600644
• UniProt ID: Q15223