Active Recombinant Human PVRL1 protein, Fc-tagged

• Cat.No.: PVRL1-0568H
• Product Overview: Recombinant Human PVRL1 protein(Gln31-Thr334), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 31-334 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Nectin-3, His Tag at 5 μg/mL (100 μL/well) can bind Human Nectin-1, Fc Tag with a linear range of 0.6-20 ng/mL. Immobilized Biotinylated Human Nectin-4, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human Nectin-1, Fc Tag with a linear range of 2-40 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 60.4 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln31-Thr334
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVRL1 poliovirus receptor-related 1 (herpesvirus entry mediator C) [ Homo sapiens ]
• Official Symbol: PVRL1
• Synonyms: PVRL1; poliovirus receptor-related 1 (herpesvirus entry mediator C); ED4, HVEC; poliovirus receptor-related protein 1; CD111; CLPED1; HIgR; nectin; OFC7; PRR; PRR1; PVRR1; SK 12; nectin 1; poliovirus receptor-like 1; herpesvirus Ig-like receptor; herpes virus entry mediator C; ectodermal dysplasia 4 (Margarita Island type); ED4; HVEC; PVRR; SK-12; nectin-1; MGC16207; MGC142031
• Gene ID: 5818
• mRNA Refseq: NM_002855
• Protein Refseq: NP_002846
• MIM: 600644
• UniProt ID: Q15223