Active Recombinant Human PSMA protein, His-Avi-tagged, Biotinylated

• Cat.No.: PSMA-0649H
• Product Overview: Biotinylated Recombinant Human PSMA protein(Lys44-Ala750), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 44-750 a.a.
• Form: Supplied as 0.2 μm filtered solution in 25 mM MES, 500 mM NaCl, pH6.5 with trehalose as protectant.
• Bio-activity: Immobilized Monoclonal Anti-Human PSMA Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human PSMA, His,Avitag, premium grade with a linear range of 0.2-6 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 83.1 kDa. The protein migrates as 95-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Lys44-Ala750
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.