Active Recombinant Human PSMA protein, Fc-tagged, SEC-HPLC verified

• Cat.No.: PSMA-0644H
• Product Overview: Active Recombinant Human PSMA protein(Q04609-1)(Lys44-Ala750) is expressed from HEK293 with hFc tag at the N-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Lys44-Ala750
• Tag: N-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PSMA, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-PSMA Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 106.8 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.