Active Recombinant Human PPP1CB, GST-tagged

• Cat.No.: PPP1CB-1515H
• Product Overview: Recombinant full-length human PP1B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-327 a.a.
• Description: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 272nmol/min/mg
• Molecular Mass: ~60 kDa
• Purity: >70%
• Applications: Phosphatase Assay, Western Blot
• Stability: 1 yr at -70°C from date of shipment.
• Storage: Store product at –70ºC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PPP1CB protein phosphatase 1, catalytic subunit, beta isozyme [ Homo sapiens (human) ]
• Official Symbol: PPP1CB
• Synonyms: PPP1CB; protein phosphatase 1, catalytic subunit, beta isozyme; protein phosphatase 1, catalytic subunit, beta isoform; serine/threonine-protein phosphatase PP1-beta catalytic subunit; PP 1B; PP1beta; protein phosphatase 1-beta; protein phosphatase 1-delta; protein phosphatase 1, catalytic subunit, delta isoform; serine/threonine protein phosphatase PP1-beta catalytic subunit; PP-1B; PPP1CD; MGC3672
• Gene ID: 5500
• mRNA Refseq: NM_002709
• Protein Refseq: NP_002700
• MIM: 600590
• UniProt ID: P62140
• Chromosome Location: 2p23
• Pathway: Adrenergic signaling in cardiomyocytes; Amphetamine addiction; Downregulation of TGF-beta receptor signaling
• Function: metal ion binding; myosin phosphatase activity; myosin-light-chain-phosphatase activity