Active Recombinant Human PNMT Protein, His-tagged

• Cat.No.: PNMT-01H
• Product Overview: Recombinant Human PNMT Protein (Met1-Leu282) with a C-terminal 6-His tag was expressed in E. coli.

Specification

• Description: The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Bio-activity: Measured by its S-Adenosyl-L-Methionine (SAM)-dependent ability to transfer a methyl group to norepinephrine. The specific activity is >2.5 pmol/min/μg.
• Molecular Mass: 32 kDa
• N-terminal Sequence Analysis: Ser2
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, EDTA, Brij-35 and Glycerol.
• Shipping: The product is shipped with polar packs.
• Protein length: 1-282 a.a.

Gene Information

• Gene Name: PNMT phenylethanolamine N-methyltransferase [ Homo sapiens (human) ]
• Official Symbol: PNMT
• Synonyms: PNMT; phenylethanolamine N-methyltransferase; PENT; PNMTase; phenylethanolamine N-methyltransferase; noradrenaline N-methyltransferase; phenylethanolamine N-methylase; EC 2.1.1.28
• Gene ID: 5409
• mRNA Refseq: NM_002686
• Protein Refseq: NP_002677
• MIM: 171190
• UniProt ID: P11086